Osteonet
In vitro 3D cell models of healthy and OSTEOpathological ageing bone tissue for implantation and drug testing in a multidisciplinary NETwork.
OSTEONET KEY OBJECTIVES
Cryopreserve the 3D constructs
Development of cryopreserved 3D constructs mimicking properties of healthy and aged bone
Develop scaffolds
Development of biomimetic and biocompatible 3D annular scaffolds
Develop bioreactor
Development of controlled dynamic bioreactor prototypes
Training
Training of dedicated technical and biological/medical personnel in dynamic cell cultures
TRANSFER OF KNOWLEDGE
Project management
Ensure efficient Project management as a guarantee for effective progress towards set objectives. Ensure that objectives are clearly defined and visible throughout the Project. Monitor Project progress & consistence between planned & actual activities. Ensure that the ToK plan is followed. Ensure that EC Project reports, deliverables & milestones are fulfilled
Lead beneficiary
Design, manufacturing and characterization of biomimetic scaffolds and constructs
Characterization of healthy and ageing bone ECM structure by μCT and image analysis. Design and manufacturing of 3D printed porous scaffolds mimicking healthy and ageing bone ECM structure. Functionalization of scaffold pore surface with osteogenic biochemical cues. Characterization of scaffolds biocompatibility
Lead beneficiary
Preparation and cryopreservation of cellular 3D constructs
Development of optimal cell seeding in the annular 3D porous constructs prepared in WP2. Definition of preconditioning protocols to guide cells to organize and differentiate in the produced scaffolds similar to healthy and ageing bone. Development of techniques to cryopreserve the produced annular 3D cellular scaffolds with minimal damages
Lead beneficiary
Design and production of dynamic bioreactors as in vitro models
Development of in silico transport models of momentum and relevant solutes in bioreactors for culture of 3D bone constructs. Design of dynamic bioreactors for culturing 3D bone constructs in controlled biochemical and fluid-mechanical microenvironment. Optimization of bioreactors design to guide 3D bone construct structure to mimic healthy and ageing bone
Lead beneficiary
IPR and exploitation
Definition of the exploitable results. IP Development and management. Finalization of the IPR agreements. Business modelling and development of new spin-offs, start-ups
Lead beneficiary
Dissemination and Communication
Communicate and disseminate the OSTEONET objectives, methods & results to stakeholders. Integrate dissemination and communication, developing an adequate strategy and defining the tools to be used, to achieve the highest level of interest throughout the project duration. Maximize the impact of research, development and demonstration, and to ensure that the results are effectively communicated.
Lead beneficiary